Document Type

Article

Publication Date

3-2003

Publication Title

Applied and Environmental Microbiology

Volume

69

Issue

3

Pages

1844-1846

DOI

10.1128/AEM.69.3.1844-1846.2003

Abstract

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Original Publication Citation

Heller, L. C., Davis, C. R., Peak, K. K., Wingfield, D., Cannons, A. C., Amuso, P. T., & Cattani, J. (2003). Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR. Applied and Environmental Microbiology, 69(3), 1844-1846. doi:10.1128/AEM.69.3.1844-1846.2003

ORCID

0000-0001-8084-0787 (Heller, L.)