The easy accessibility of skin makes it an excellent target for gene transfer protocols. To take advantage of skin as a target for gene transfer, it is important to establish an efficient and reproducible delivery system. Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo. A critical component of this technique is the electrode configuration. Electroporation parameters were optimized for transgene expression with minimal tissue damage with a novel electrode. The highest transgene expression and efficiency of individual cell transformation with minimal damage was produced with eight 150 ms pulses at field strength of 100 V/cm. This electrode design offers the potential for easier and more reproducible electrically mediated cutaneous plasmid delivery than the simple electrodes currently commercially available. This electrode can be a valuable tool in determining the applicability of electrically mediated cutaneous gene transfer.
Original Publication Citation
Heller, L. C., Jaroszeski, M. J., Coppola, D., McCray, A. N., Hickey, J., & Heller, R. (2007). Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode. Gene Therapy, 14(3), 275-280. doi:10.1038/sj.gt.3302867
Heller, L. C.; Jaroszeski, M. J.; Coppola, D.; McCray, A. N.; Hickey, J.; and Heller, R., "Optimization of Cutaneous Electrically Mediated Plasmid DNA Delivery Using Novel Electrode" (2007). Bioelectrics Publications. 109.
0000-0001-8084-0787 (Heller, L.), 0000-0003-1899-3859 (Heller, R.)