Proceedings of the National Academy of Sciences
Mycoplasma pneumoniae (Mp) infections cause tracheobronchitis and "walking" pneumonia, and are linked to asthma and other reactive airway diseases. As part of the infectious process, the bacterium expresses a 591-aa virulence factor with both mono-ADP ribosyltransferase (mART) and vacuolating activities known as Community-Acquired Respiratory Distress Syndrome Toxin (CARDS TX). CARDS TX binds to human surfactant protein A and annexin A2 on airway epithelial cells and is internalized, leading to a range of pathogenetic events. Here we present the structure of CARDS TX, a triangular molecule in which N-terminal mART and C-terminal tandem beta-trefoil domains associate to form an overall architecture distinct from other well-recognized ADP-ribosylating bacterial toxins. We demonstrate that CARDS TX binds phosphatidylcholine and sphingomyelin specifically over other membrane lipids, and that cell surface binding and internalization activities are housed within the C-terminal beta-trefoil domain. The results enhance our understanding of Mp pathogenicity and suggest a novel avenue for the development of therapies to treat Mp-associated asthma and other acute and chronic airway diseases.
Original Publication Citation
Becker, A., Kannan, T.R., Taylor, A.B., Pakhomova, O.N., Zhang, Y., Somarajan, S.R., . . . Hart, P.J. (2015). Structure of CARDS toxin, a unique ADP-ribosylating and vacuolating cytotoxin from Mycoplasma pneumoniae. Proceedings of the National Academy of Sciences, 112(16), 5165-5170. doi: 10.1073/pnas.1420308112
Becker, Argentina; Kannan, T. R.; Taylor, Alexander B.; Pakhomova, Olga N.; Zhang, Yanfeng; Somarajan, Sudha R.; Galaleldeen, Ahmad; Holloway, Stephen P.; Baseman, Joel B.; and Hart, P. John, "Structure of CARDS toxin, a unique ADP-ribosylating and vacuolating Cytotoxin from Mycoplasma Pneumoniae" (2015). Bioelectrics Publications. 27.