Physical Review E
4, Pt. 1
Numerical simulations for electrically induced, intracellular calcium release from the endoplasmic reticulum are reported. A two-step model is used for self-consistency. Distributed electrical circuit representation coupled with the Smoluchowski equation yields the ER membrane nanoporation for calcium outflow based on a numerical simulation. This is combined with the continuum Li-Rinzel model and drift diffusion for calcium dynamics. Our results are shown to be in agreement with reported calcium release data. A modest increase (rough doubling) of the cellular calcium is predicted in the absence of extra-cellular calcium. In particular, the applied field of 15 kV/cm with 60 ns pulse duration makes for a strong comparison. No oscillations are predicted and the net recovery period of about 5 min are both in agreement with published experimental results. A quantitative explanation for the lack of such oscillatory behavior, based on the density dependent calcium fluxes, is also provided.
Original Publication Citation
Joshi, R.P., Nguyen, A., Sridhara, V., Hu, Q., Nuccitelli, R., Beebe, S.J., . . . Schoenbach, K.H. (2007). Simulations of intracellular calcium release dynamics in response to a high-intensity, ultrashort electric pulse. Phys Rev E Stat Nonlin Soft Matter Phys, 75(4 Pt 1), 041920. doi:10.1103/PhysRevE.75.041920
Joshi, R. P.; Nguyen, A.; Sridhara, V.; Hu, Q.; Nuccitelli, R.; Beebe, Stephen J.; Kolb, J.; and Schoenbach, Karl H., "Simulations of Intracellular Calcium Release Dynamics in Response to a High-Intensity, Ultrashort Electric Pulse" (2007). Bioelectrics Publications. 42.