Differential Dielectric Responses of Chondrocyte and Jurkat Cells in Electromanipulation Buffers

Document Type

Article

Publication Date

2015

Publication Title

Platelets

Volume

26

Issue

1

Pages

13-16

DOI

10.1002/elps.201500119

Abstract

Electromanipulation of cells as a label-free cell manipulation and characterization tool has gained particular interest recently. However, the applicability of electromanipulation, particularly dielectrophoresis (DEP), to biological cells is limited to cells suspended in buffers containing lower amounts of salts relative to the physiological buffers. One might question the use of low conductivity buffers (LCBs) for DEP separation, as cells are stressed in buffers lacking physiological levels of salt. In LCB, cells leak ions and undergo volume regulation. Therefore, cells exhibit time-dependent DEP response in LCB. In this work, cellular changes in LCB are assessed by dielectric spectroscopy, cell viability assay, and gene expression of chondrocytes and Jurkats. Results indicate leakage of ions from cells, increases in cytoplasmic conductivity, membrane capacitance, and conductance. Separability factor, which defines optimum conditions for DEP cell separation, for the two cell types is calculated using the cellular dielectric data. Optimum DEP separation conditions change as cellular dielectric properties evolve in LCB. Genetic analyses indicate no changes in expression of ionic channel proteins for chondrocytes suspended in LCB. Retaining cellular viability might be important during dielectrophoretic separation, especially when cells are to be biologically tested at a downstream microfluidic component.

Original Publication Citation

Sabuncu, A.C., Asmar, A.J., Stacey, M.W., & Beskok, A. (2015). Differential dielectric responses of chondrocyte and jurkat cells in electromanipulation buffers. Electrophoresis, 36(13), 1499-1506. doi: 10.1002/elps.201500119

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