Document Type

Article

Publication Date

2023

DOI

10.1016/j.xpro.2023.102703

Publication Title

STAR Protocols

Volume

4

Issue

4

Pages

102703 (1-13)

Abstract

Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications.

For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).1

*1 Liu, Q., Li, C., Deng, B., Gao, P., Wang, L., Li, Y., ... & Fu, X. (2023). Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development. Cell reports, 42(3) 112166. https://doi.org/10.1016/j.celrep.2023.112166

Rights

© 2023 The Authors.

This is an open access article, published under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International (CC BY-NC-ND 4.0) License.

Data Availability

Article states: "This protocol did not generate new datasets, nor data analyzing codes."

Original Publication Citation

Liu, Q., Li, C., Li, Y., Wang, L., Zhang, X., Deng, B., Gao, P., Shiri, M., Alkaifi, F., Zhao, J., Stephens, J. M., Simintiras, C. A., Francis, J., Sun, J., & Fu, X. (2023). Progenitor cell isolation from mouse epididymal adipose tissue and sequencing library construction. STAR Protocols, 4(4), 1-13, Article 102703. https://doi.org/10.1016/j.xpro.2023.102703

ORCID

0009-0000-8905-7553 (Sun)

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