Date of Award

Fall 2006

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Electrical & Computer Engineering

Program/Concentration

Electrical Engineering

Committee Director

Karl H. Schoenbach

Committee Member

Juergen F. Kolb

Committee Member

Ravindra P. Joshi

Call Number for Print

Special Collections LD4331.E55 S54 2006

Abstract

Nanosecond pulsed electric fields are able to manipulate internal cell organelles by taking advantage of the small but finite time required for charge to establish across a capacitive barrier. Pulsed electric fields also affect macromolecular structures such as enzymes and proteins. Application of a single nanosecond pulsed electric field decreases the fluorescence of a commercially available biological dye Bodipy FL C5 Ceramide, used to observe the Golgi apparatus of living cells. The effect is seen when the fluorophore is exposed to an electric field both in vitro and when incorporated into the Golgi of a living Jurkat cell. Observation with a laser scanning confocal microscope provides high resolution thin slices for analysis. This indicates it may be possible to alter the structure and function of other biologically significant molecules both in vitro and in situ for a variety of applications. No changes in the gross morphology of the Golgi were observed after cells or dye was exposed to the electric field.

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DOI

10.25777/6b88-np94

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