Document Type

Article

Publication Date

2024

DOI

10.1016/j.resp.2024.104283

Publication Title

Respiratory Physiology & Neurobiology

Volume

326

Pages

104283 (1-7)

Abstract

The utilization of continuous wave (CW) near-infrared spectroscopy (NIRS) device to measure non-invasively muscle oxygenation in healthy and disease states is limited by the uncertainties related to the differential path length factor (DPF). DPF value is required to quantify oxygenated and deoxygenated heme groups' concentration changes from measurement of optical densities by NIRS. An integrated approach that combines animal and computational models of oxygen transport and utilization was used to estimate the DPF value in situ. The canine model of muscle oxidative metabolism allowed measurement of both venous oxygen content and tissue oxygenation by CW NIRS under different oxygen delivery conditions. The experimental data obtained from the animal model were integrated in a computational model of O2 transport and utilization and combined with Beer-Lambert law to estimate DPF value in contracting skeletal muscle. A 2.1 value was found for DPF by fitting the mathematical model to the experimental data obtained in contracting muscle (T3) (Med.Sci.Sports.Exerc.48 (10):2013-2020,2016). With the estimated value of DPF, model simulations well predicted the optical density measured by NIRS on the same animal model but with different blood flow, arterial oxygen contents and contraction rate (J.Appl.Physiol.108:1169-1176, 2010 and 112:9-19,2013) and demonstrated the robustness of the approach proposed in estimating DPF value. The approach used can overcome the semi-quantitative nature of the NIRS and estimate non-invasively DPF to obtain an accurate concentration change of oxygenated and deoxygenated hemo groups by CW NIRS measurements in contracting skeletal muscle under different oxygen delivery and contraction rate.

Rights

© 2024 The Authors.

This is an open access article under the Creative Commons Attribution 4.0 International (CC BY 4.0) License.

Data Availability

Article states: "Data will be made available on request."

Original Publication Citation

Koirala, B., Concas, A., Cincotti, A., Sun, Y., Hernández, A., Goodwin, M. L., Gladden, L. B., & Lai, N. (2024). Estimation of differential pathlength factor from NIRS measurement in skeletal muscle. Respiratory Physiology & Neurobiology, 326, 1-7, Article 104283. https://doi.org/10.1016/j.resp.2024.104283

ORCID

0000-0002-3436-289X (Lai)

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