Document Type

Article

Publication Date

2016

DOI

10.14814/phy2.12890

Publication Title

Physiological Reports

Volume

4

Issue

15

Pages

e12890 (1-9)

Abstract

The study aims to characterize age-associated changes in skeletal muscle bioenergetics by evaluating the response to ischemia-reperfusion in the skeletal muscle of the Goto-Kakizaki (GK) rats, a rat model of non-obese type 2 diabetes (T2D). 31P magnetic resonance spectroscopy (MRS) and blood oxygen level-dependent (BOLD) MRI was performed on the hindlimb of young (12 weeks) and adult (20 weeks) GK and Wistar (control) rats. 31P-MRS and BOLD-MRI data were acquired continuously during an ischemia and reperfusion protocol to quantify changes in phosphate metabolites and muscle oxygenation. The time constant of phosphocreatine recovery, an index of mitochondrial oxidative capacity, was not statistically different between GK rats (60.8 ± 13.9 sec in young group, 83.7 ± 13.0 sec in adult group) and their age-matched controls (62.4 ± 11.6 sec in young group, 77.5 ± 7.1 sec in adult group). During ischemia, baseline-normalized BOLD-MRI signal was significantly lower in GK rats than in their age-matched controls. These results suggest that insulin resistance leads to alterations in tissue metabolism without impaired mitochondrial oxidative capacity in GK rats. © 2016 The Authors.

Comments

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license.

https://creativecommons.org/licenses/by/3.0/

Original Publication Citation

Liu, Y., Mei, X., Li, J., Lai, N., & Yu, X. (2016). Mitochondrial function assessed by 31P MRS and BOLD MRI in non-obese type 2 diabetic rats. Physiological Reports, 4(15), e12890. doi:10.14814/phy2.12890

ORCID

0000-0002-3436-289X (Lai)