Event Title

Characterization of a Toxin-Antitoxin Locus in Acinetobacter baumannii

Student Presenter Information

Michaela Frost, Old Dominion University

Presentation Type

Event

Disciplines

Biology | Microbiology

Description/Abstract

Toxin-antitoxin (TA) systems are mechanisms of survival in many species of bacteria. In nontypeable Haemophilus influenzae (NTHi), the type II TA gene pair vapBC-1 encodes the VapB-1 antitoxin protein and the VapC-1 ribonuclease toxin. This locus contributes significantly to NTH’s survival and virulence during infection. Orthologues of vapBC-1 are present in the Gram-negative bacillus Acinetobacter baumannii, a causative agent of nosocomial infections. In this study, we hypothesized that protein homologues of VapB- 1 and VapC-1 in A. baumannii would interact similarly as those in NTHi. We also investigated whether the VapCAb toxin protein had ribonuclease activity and led to growth arrest.

Comments

Faculty Mentor: Dr. Dayle Daines

Location

Learning Commons @ Perry Library, Conference Room 1310

Start Date

13-2-2016 11:30 AM

End Date

13-2-2016 12:30 PM

Full Text of Presentation

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Feb 13th, 11:30 AM Feb 13th, 12:30 PM

Characterization of a Toxin-Antitoxin Locus in Acinetobacter baumannii

Learning Commons @ Perry Library, Conference Room 1310

Toxin-antitoxin (TA) systems are mechanisms of survival in many species of bacteria. In nontypeable Haemophilus influenzae (NTHi), the type II TA gene pair vapBC-1 encodes the VapB-1 antitoxin protein and the VapC-1 ribonuclease toxin. This locus contributes significantly to NTH’s survival and virulence during infection. Orthologues of vapBC-1 are present in the Gram-negative bacillus Acinetobacter baumannii, a causative agent of nosocomial infections. In this study, we hypothesized that protein homologues of VapB- 1 and VapC-1 in A. baumannii would interact similarly as those in NTHi. We also investigated whether the VapCAb toxin protein had ribonuclease activity and led to growth arrest.