Event Title

Use of Unionized Ammonia to Control Zooplankton Grazers in Cultures of Scenedesmus sp.

Student Presenter Information

Blake Steiner, Old Dominion University
BRL Team

Presentation Type

Event

Description/Abstract

An experiment was conducted to limit Scenedesmus sp. grazers, as they continue to reduce biofuel yields. 2.5 L cultures of Scenedesmus sp. with zooplankton were inoculated in photobioreactors with 640 mg/L urea. 500 mg/L spike of NH4Cl was used on day zero to test use of free ammonia to control grazers. Cell counts performed with hemocytometer and Gridded Sedgewick Rafter 1 mm2. Ammonia was measured using ion chromatography. Urea may impact zooplankton by day 8 out of 14, with 24-48 hour lag time after accumulating free ammonia. This was confirmed by NH4Cl spike.

Comments

Mentor: Dr. Sandeep Kumar

Location

Learning Commons @ Perry Library, Conference Room 1310

Start Date

18-2-2017 9:00 AM

End Date

18-2-2017 10:00 AM

Full Text of Presentation

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Feb 18th, 9:00 AM Feb 18th, 10:00 AM

Use of Unionized Ammonia to Control Zooplankton Grazers in Cultures of Scenedesmus sp.

Learning Commons @ Perry Library, Conference Room 1310

An experiment was conducted to limit Scenedesmus sp. grazers, as they continue to reduce biofuel yields. 2.5 L cultures of Scenedesmus sp. with zooplankton were inoculated in photobioreactors with 640 mg/L urea. 500 mg/L spike of NH4Cl was used on day zero to test use of free ammonia to control grazers. Cell counts performed with hemocytometer and Gridded Sedgewick Rafter 1 mm2. Ammonia was measured using ion chromatography. Urea may impact zooplankton by day 8 out of 14, with 24-48 hour lag time after accumulating free ammonia. This was confirmed by NH4Cl spike.