Event Title

Non-lethal Assay for Detecting Mycobacterial Exposure in Striped Bass (Morone saxatilis)

Location

Learning Commons @ Perry Library, Northwest Atrium

Start Date

18-2-2017 8:00 AM

End Date

18-2-2017 12:30 PM

Description

Mycobacterium (M.) marinum is a known pathogen that infects a number of aquatic species. This pathogen is greatly affecting the Chesapeake Bay fisheries. In this study, we looked at Morone saxatilis (striped bass) that were infected with M. marinum species (spp) to detect an immune response against M. marinum. In previous studies, the only means to detect if striped bass were infected with M. marinum that did not express clinical signs, such as visible lesions or granulomas, was by euthanizing and dissecting their spleens. This study aims to prevent future lethal sampling of fishes to detect infection by taking blood samples. To do so, we test the sera of their blood to detect immune antibody responses to proteins of M. marinum by Western Blot analysis.

Comments

Mentor: Dr. David Gauthier
Biological Sciences

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Feb 18th, 8:00 AM Feb 18th, 12:30 PM

Non-lethal Assay for Detecting Mycobacterial Exposure in Striped Bass (Morone saxatilis)

Learning Commons @ Perry Library, Northwest Atrium

Mycobacterium (M.) marinum is a known pathogen that infects a number of aquatic species. This pathogen is greatly affecting the Chesapeake Bay fisheries. In this study, we looked at Morone saxatilis (striped bass) that were infected with M. marinum species (spp) to detect an immune response against M. marinum. In previous studies, the only means to detect if striped bass were infected with M. marinum that did not express clinical signs, such as visible lesions or granulomas, was by euthanizing and dissecting their spleens. This study aims to prevent future lethal sampling of fishes to detect infection by taking blood samples. To do so, we test the sera of their blood to detect immune antibody responses to proteins of M. marinum by Western Blot analysis.