Non-lethal Assay for Detecting Mycobacterial Exposure in Striped Bass (Morone saxatilis)
Description/Abstract/Artist Statement
Mycobacterium (M.) marinum is a known pathogen that infects a number of aquatic species. This pathogen is greatly affecting the Chesapeake Bay fisheries. In this study, we looked at Morone saxatilis (striped bass) that were infected with M. marinum species (spp) to detect an immune response against M. marinum. In previous studies, the only means to detect if striped bass were infected with M. marinum that did not express clinical signs, such as visible lesions or granulomas, was by euthanizing and dissecting their spleens. This study aims to prevent future lethal sampling of fishes to detect infection by taking blood samples. To do so, we test the sera of their blood to detect immune antibody responses to proteins of M. marinum by Western Blot analysis.
Presentation Type
Event
Location
Learning Commons @ Perry Library, Northwest Atrium
Start Date
2-18-2017 8:00 AM
End Date
2-18-2017 12:30 PM
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Non-lethal Assay for Detecting Mycobacterial Exposure in Striped Bass (Morone saxatilis)
Learning Commons @ Perry Library, Northwest Atrium
Mycobacterium (M.) marinum is a known pathogen that infects a number of aquatic species. This pathogen is greatly affecting the Chesapeake Bay fisheries. In this study, we looked at Morone saxatilis (striped bass) that were infected with M. marinum species (spp) to detect an immune response against M. marinum. In previous studies, the only means to detect if striped bass were infected with M. marinum that did not express clinical signs, such as visible lesions or granulomas, was by euthanizing and dissecting their spleens. This study aims to prevent future lethal sampling of fishes to detect infection by taking blood samples. To do so, we test the sera of their blood to detect immune antibody responses to proteins of M. marinum by Western Blot analysis.
Comments
Mentor: Dr. David Gauthier
Biological Sciences