Reconstructing three dimensional structures (3DR) from histological sections has always been difficult but is becoming more accessible with the assistance of digital imaging. We sought to assemble a low cost system using readily available hardware and software to generate 3DR for a study of tadpole chondrocrania. We found that a combination of RGB can1era, stereomicroscope, and Apple Macintosh PowerPC computers running NIH Image, Object Image, Rotater, and SURFdriver software provided acceptable reconstructions. These are limited in quality primarily by the distortions arising from histological protocols rather than hardware or software.
Radice, Gary; Boggiano, Mary K.; DeSantis, Mark; Larson, Peter; Oppong, Joseph; Smetanick, Matthew; Stevens, Todd; Tripp, James; Weber, Rebecca; Kerckhove, Michael; and Sá, Rafael de
"Three-Dimensional Reconstructions of Tadpole Chondrocrania from Historical Sections,"
Virginia Journal of Science: Vol. 50
, Article 1.
Available at: http://digitalcommons.odu.edu/vjs/vol50/iss3/1