Applied and Environmental Microbiology
In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.
Original Publication Citation
Heller, L. C., Davis, C. R., Peak, K. K., Wingfield, D., Cannons, A. C., Amuso, P. T., & Cattani, J. (2003). Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR. Applied and Environmental Microbiology, 69(3), 1844-1846. doi:10.1128/AEM.69.3.1844-1846.2003
Heller, Loree C.; Davis, Carisa R.; Peak, K. Kealy; Wingfield, David; Cannons, Andrew C.; Amuso, Philip T.; and Cattani, Jacqueline, "Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia Coli by Real-Time PCR" (2003). Bioelectrics Publications. 107.
0000-0001-8084-0787 (Heller, L.)