Document Type
Article
Publication Date
2018
Publication Title
Cancers
Volume
10
Issue
3
Pages
69 (19 pages)
DOI
10.3390/cancers10030069
Abstract
Nano-pulse stimulation (NPS), previously called nsPEFs, induced a vaccine-like effect after ablation of orthotopic N1-S1 hepatocellular carcinoma (HCC), protecting rats from subsequent challenges with N1-S1 cells. To determine immunity, immune cell phenotypes were analyzed in naïve, treated and protected rats. NPS provides a positive, post-ablation immuno-therapeutic outcome by alleviating immunosuppressive T regulatory cells (Treg) in the tumor microenvironment (TME), allowing dendritic cell influx and inducing dynamic changes in natural killer cells (NKs), NKT-cells and T-lymphocytes in blood, spleen and liver. NPS induced specific increases in NKs and NKT-cells expressing CD8 and activation receptors CD314-NKG2D and CD161 (NK1.1) in the TME after treatment, as well as some variable changes in CD4+ and CD8+ effector (Tem) and central memory (Tem) lymphocytes in blood and spleen. After orthotopic challenge, CD8+ T-cells were cytotoxic, inducing apoptosis in N1-S1 cells; additionally, in contrast to post-treatment immune responses, CD4+ and CD8+ memory precursor effector cells (MPECs) and short-lived effector cells (SLECs) were present, while still including CD8+ CD161 NK cells, but not involving CD8+ CD314-NKG2D+ NKs. This immunity was N1-S1-specific and was sustained for at least 8 months. NPS vaccinates rats in vivo against HCC by activating innate and adaptive immune memory mechanisms that prevent HCC recurrence.
Original Publication Citation
Lassiter, B. P., Guo, S., & Beebe, S. J. (2018). Nano-pulse stimulation ablates orthotopic rat hepatocellular carcinoma and induces innate and adaptive memory immune mechanisms that prevent recurrence. Cancers,10(3), 69. doi:http://dx.doi.org/10.3390/cancers10030069
Repository Citation
Lassiter, Brittany P.; Guo, Siqi; and Beebe, Stephen J., "Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma and Induces Innate and Adaptive Memory Immune Mechanisms that Prevent Recurrence" (2018). Bioelectrics Publications. 163.
https://digitalcommons.odu.edu/bioelectrics_pubs/163
ORCID
0000-0002-7280-7888 (Guo), 0000-0002-6075-9452 (Beebe)
Included in
Bioelectrical and Neuroengineering Commons, Cell Biology Commons, Medical Biotechnology Commons, Oncology Commons
Comments
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.