Document Type

Article

Publication Date

2009

Publication Title

Biochimica et Biophysica Acta

Volume

1788

Issue

5

Pages

1168-1175

DOI

10.1016/j.bbamem.2009.02.006

Abstract

Changes in [Ca2+]i response of individual Jurkat cells to nanosecond pulsed electric fields (nsPEFs) of 60 ns and field strengths of 25, 50, and 100 kV/cm were investigated. The magnitude of the nsPEF-induced rise in [Ca2+]i was dependent on the electric field strength. With 25 and 50 kV/cm, the [Ca2+]i response was due to the release of Ca2+ from intracellular stores and occurred in less than 18 ms. With 100 kV/cm, the increase in [Ca2+]i was due to both internal release and to influx across the plasma membrane. Spontaneous changes in [Ca2+]i exhibited a more gradual increase over several seconds. The initial, pulse-induced [Ca2+]i response initiates at the poles of the cell with respect to electrode placement and co-localizes with the endoplasmic reticulum. The results suggest that nsPEFs target both the plasma membrane and subcellular membranes and that one of the mechanisms for Ca2+ release may be due to nanopore formation in the endoplasmic reticulum.

Comments

Web of Science: "Free full-text from publisher -- gold open access."

Original Publication Citation

Scarlett, S. S., White, J. A., Blackmore, P. F., Schoenbach, K. H., & Kolb, J. F. (2009). Regulation of intracellular calcium concentration by nanosecond pulsed electric fields. Biochimica et Biophysica Acta, 1788(5), 1168-1175. doi:10.1016/j.bbamem.2009.02.006

ORCID

0000-0001-7867-7773 (Schoenbach), 0000-0002-0434-5001 (Kolb)

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