Antimicrobial Agents and Chemotherapy
e00744-18 (9 pages)
Antimicrobial lock solutions are important for prevention of microbial colonization and infection of long-term central venous catheters. We investigated the efficacy and safety of a novel antibiotic-free lock solution formed from gas plasma-activated disinfectant (PAD). Using a luminal biofilm model, viable cells of methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans in mature biofilms were reduced by 6 to 8 orders of magnitude with a PAD lock for 60 min. Subsequent 24-h incubation of PAD-treated samples resulted in no detectable regrowth of viable bacteria or fungi. As a comparison, the use of a minocycline-EDTA-ethanol lock solution for 60 min led to regrowth of bacteria and fungi, up to 10(7) to 10(9) CFU/ml, in 24 h. The PAD lock solution had minimal impact on human umbilical vein endothelial cell viability, whereas the minocycline-EDTA-ethanol solution elicited cell death in nearly half of human endothelial cells. Additionally, PAD treatment caused little topological change to catheter materials. In conclusion, PAD represents a novel antibiotic-free, noncytotoxic lock solution that elicits rapid and broad-spectrum eradication of biofilm-laden microbes and shows promise for the prevention and treatment of intravascular catheter infections.
Original Publication Citation
Bhatt, S., Mehta, P., Chen, C., Daines, D. A., Mermel, L. A., Chen, H. L., & Kong, M. G. (2018). Antimicrobial efficacy and safety of a novel gas plasma-activated catheter lock solution. Antimicrobial Agents and Chemotherapy, 62(8), e00744-00718. doi:10.1128/aac.00744-18
Bhatt, Sudhir; Mehta, Poonam; Chen, Chen; Daines, Dayle A.; Mermel, Leonard A.; Chen, Hai-Lan; and Kong, Michael G., "Antimicrobial Efficacy and Safety of a Novel Gas Plasma-Activated Catheter Lock Solution" (2018). Bioelectrics Publications. 225.