Document Type

Article

Publication Date

8-2018

Publication Title

Antimicrobial Agents and Chemotherapy

Volume

62

Issue

8

Pages

e00744-18 (9 pages)

DOI

10.1128/aac.00744-18

Abstract

Antimicrobial lock solutions are important for prevention of microbial colonization and infection of long-term central venous catheters. We investigated the efficacy and safety of a novel antibiotic-free lock solution formed from gas plasma-activated disinfectant (PAD). Using a luminal biofilm model, viable cells of methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans in mature biofilms were reduced by 6 to 8 orders of magnitude with a PAD lock for 60 min. Subsequent 24-h incubation of PAD-treated samples resulted in no detectable regrowth of viable bacteria or fungi. As a comparison, the use of a minocycline-EDTA-ethanol lock solution for 60 min led to regrowth of bacteria and fungi, up to 10(7) to 10(9) CFU/ml, in 24 h. The PAD lock solution had minimal impact on human umbilical vein endothelial cell viability, whereas the minocycline-EDTA-ethanol solution elicited cell death in nearly half of human endothelial cells. Additionally, PAD treatment caused little topological change to catheter materials. In conclusion, PAD represents a novel antibiotic-free, noncytotoxic lock solution that elicits rapid and broad-spectrum eradication of biofilm-laden microbes and shows promise for the prevention and treatment of intravascular catheter infections.

Comments

Copyright © 2018 American Society for Microbiology. All Rights Reserved.

Original Publication Citation

Bhatt, S., Mehta, P., Chen, C., Daines, D. A., Mermel, L. A., Chen, H. L., & Kong, M. G. (2018). Antimicrobial efficacy and safety of a novel gas plasma-activated catheter lock solution. Antimicrobial Agents and Chemotherapy, 62(8), e00744-00718. doi:10.1128/aac.00744-18

Available for download on Friday, February 01, 2019

Share

COinS