Document Type

Article

Publication Date

2021

Publication Title

Cancers

Volume

13

Issue

5

Pages

1-14

DOI

10.3390/cancers13051042

Abstract

Muscle-invasive bladder cancer (MIBC) is a fast-growing and aggressive malignant tumor in urinary system. Since chemotherapy and immunotherapy are only useable with a few MIBC patients, the clinical treatment of MIBC still faces challenges. Here, we examined the feasibility of plasma-activated saline (PAS) as a fledgling therapeutic strategy for MIBC treatment. Our data showed that plasma irradiation could generate a variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in saline. In vivo tests revealed that pericarcinomatous tissue injection with PAS was effective at preventing subcutaneous bladder tumor growth, with no side effects to the visceral organs after long-term administration, as well as having no obvious influence on the various biochemistry indices of the blood in mice. The in vitro studies indicated that adding 30% PAS in cell culture media causes oxidative damage to the bladder transitional cells T24 and J82 through enhancing the intracellular ROS level, and eventually induces cancer cells' apoptosis by activating the ROS-mediated Fas/CD95 pathway. Therefore, for an intracavity tumor, these initial observations suggest that the soaking of the tumor tissue with PAS by intravesical perfusion may be a novel treatment option for bladder cancer.

Comments

© 2021 The Authors

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) license.

Original Publication Citation

Zhang, H., Zhang, J. S., Guo, B., Chen, H. L., Xu, D. H., & Kong, M. G. (2021). The antitumor effects of plasma-activated saline on muscle-invasive bladder cancer cells in vitro and in vivo demonstrate Its feasibility as a potential therapeutic approach. Cancers, 13(5), 1-14, Article 1042. https://doi.org/10.3390/cancers13051042

ORCID

0000-0001-8142-4726 (Kong)

COinS