Document Type

Article

Publication Date

2024

Publication Title

Scientific Reports

Volume

14

Issue

1

Pages

885 (1-12)

DOI

10.1038/s41598-024-51298-y

Abstract

Nanosecond pulsed atmospheric pressure plasma jets (ns-APPJs) produce reactive plasma species, including charged particles and reactive oxygen and nitrogen species (RONS), which can induce oxidative stress in biological cells. Nanosecond pulsed electric field (nsPEF) has also been found to cause permeabilization of cell membranes and induce apoptosis or cell death. Combining the treatment of ns-APPJ and nsPEF may enhance the effectiveness of cancer cell inactivation with only moderate doses of both treatments. Employing ns-APPJ powered by 9 kV, 200 ns pulses at 2 kHz and 60-nsPEF of 50 kV/cm at 1 Hz, the synergistic effects on pancreatic cancer cells (Pan02) in vitro were evaluated on the metabolic activities of cells and transcellular electrical resistance (TER). It was observed that treatment with ns-APPJ for > 2 min disrupts Pan02 cell stability and resulted in over 30% cell death. Similarly, applying nsPEF alone, > 20 pulses resulted in over 15% cell death. While the inactivation activity from the individual treatment is moderate, combined treatments resulted in 80% cell death, approximately 3-to-fivefold increase compared to the individual treatment. In addition, reactive oxygen species such as OH and O were identified at the plasma-liquid interface. The gas temperature of the plasma and the temperature of the cell solution during treatments were determined to be near room temperature.

Rights

© The Authors 2024

This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original authors and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

Data Availability

Article states: "All data generated or analyzed during this study are included in this paper. Any additional data or subset of datasets used for this study are available from the corresponding author upon request."

Original Publication Citation

Oshin, E. A., Minhas, Z., Colunga Biancatelli, R. M. L., Catravas, J. D., Heller, R., Guo, S., & Jiang, C. (2024). Synergistic effects of nanosecond pulsed plasma and electric field on inactivation of pancreatic cancer cells in vitro. Scientific Reports, 14(1), 1-12, Article 885. https://doi.org/10.1038/s41598-024-51298-y

ORCID

0000-0002-2932-9470 (Oshin), 0000-0002-1174-3876 (Biancatelli), 0000-0002-5098-295X (Catravas), 0000-0002-7280-7888 (Guo)

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