Physical Review E
5, Pt. 1
We provide a simple, but physical analysis for cell irreversibility and apoptosis in response to an ultrashort (nanosecond), high-intensity electric pulse. Our approach is based on an energy landscape model for determining the temporal evolution of the configurational probability function p(q). The primary focus is on obtaining qualitative predictions of a pulse width dependence to apoptotic cell irreversibility that has been observed experimentally. The analysis couples a distributed electrical model for current flow with the Smoluchowski equation to provide self-consistent, time-dependent transmembrane voltages. The model captures the essence of the experimentally observed pulse-width dependence, and provides a possible physical picture that depends only on the electrical trigger. A number of interesting features are predicted.
Original Publication Citation
Joshi, R.P., Hu, Q., Schoenbach, K.H., & Beebe, S.J. (2004). Energy-landscape-model analysis for irreversibility and its pulse-width dependence in cells subjected to a high-intensity ultrashort electric pulse. Phys Rev E Stat Nonlin Soft Matter Phys, 69(5 Pt 1), 051901. doi:10.1103/PhysRevE.69.051901
Joshi, R. P.; Hu, Q.; Schoenbach, Karl H.; and Beebe, Stephen J., "Energy-Landscape-Model Analysis for Irreversibility and its Pulse-Width Dependence in Cells Subjected to a High-Intensity Ultrashort Electric Pulse" (2004). Bioelectrics Publications. 38.