Molecular Therapy -- Methods & Clinical Development
Plasma-activated air (PAA) provides a noncontact DNA transfer platform. In the current study, PAA was used for the delivery of plasmid DNA in a 3D human skin model, as well as in vivo. Delivery of plasmid DNA encoding luciferase to recellularized dermal constructs was enhanced, resulting in a fourfold increase in luciferase expression over 120 hours compared to injection only (P < 0.05). Delivery of plasmid DNA encoding green fluorescent protein (GFP) was confirmed in the epidermal layers of the construct. In vivo experiments were performed in BALB/c mice, with skin as the delivery target. PAA exposure significantly enhanced luciferase expression levels 460-fold in exposed sites compared to levels obtained from the injection of plasmid DNA alone (P < 0.001). Expression levels were enhanced when the plasma reactor was positioned more distant from the injection site. Delivery of plasmid DNA encoding GFP to mouse skin was confirmed by immunostaining, where a 3-minute exposure at a 10 mm distance displayed delivery distribution deep within the dermal layers compared to an exposure at 3 mm where GFP expression was localized within the epidermis. Our findings suggest PAA-mediated delivery warrants further exploration as an alternative approach for DNA transfer for skin targets.
Original Publication Citation
Edelblute, C. M., Heller, L. C., Malik, M. A., Bulysheva, A., & Heller, R. (2016). Plasma-activated air mediates plasmid DNA delivery in vivo. Molecular Therapy — Methods & Clinical Development, 3, 16028. doi: 10.1038/mtm.2016.28
Edelblute, Chelsea M.; Heller, Loree C.; Malik, Muhammad A.; Bulysheva, Anna; and Heller, Richard, "Plasma-Activated Air Mediates Plasmid DNA Delivery In Vivo" (2016). Bioelectrics Publications. 88.