Date of Award

Summer 1977

Document Type


Degree Name

Master of Science (MS)


Biological Sciences



Committee Director

A. J. Ward

Committee Member

A. H. Johnson

Committee Member

H. D. Whitten

Call Number for Print

Special Collections LD4331.B46 M352


Mycelial (M) and blastopore (B) cell homogenates of Candida albicans strain BJ85 were compared to detect and recover unique M components. Qualitative differences in the protein and lipid content of Mand B homogenates were suggested by discontinuous polyacrylamide gel electrophoresis (DPAGE). In addition, a quantitative and possibly qualitative shift in the protein content during germ tube formation was implied by DPAGE. Unique M components were detected using both DPAGE and immunoelectrophoretic (IE) techniques. One unique M component by DPAGE, four by IE, and two by combined DPAGE and IE techniques were suggested. Of these three methods, only DPAGE and the coI11bined DP.AGE and IE techniques were suitable for the quantitative recovery of unique components. Of these two, the combined DPAGE and IE techniques seem to have greater potential for the detection of unique M components.


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