Document Type

Article

Publication Date

2018

DOI

10.1002/aps3.1142

Publication Title

Applications in Plant Sciences

Volume

6

Issue

4

Pages

e1142 (5 pages)

Abstract

PREMISE OF THE STUDY: Few genetic markers provide phylogenetic information in closely related species of Isoëtes (Isoëtaceae). We describe the development of primers for several putative low- copy nuclear markers to resolve the phylogeny of Isoëtes, particularly in the southeastern United States.

METHODS AND RESULTS: We identified regions of interest in Isoëtes transcriptomes based on low- copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of Isoëtes and one species of Lycopodium. Parts of the pgiC, gapC, and IBR3 gene regions show phylogenetic signal within the North American and Mediterranean clades of Isoëtes.

CONCLUSIONS: Transcriptome data prove useful for identification and primer design of low-copy genes. Three new markers show potential for inferring phylogenies in regional clades of Isoëtes, and possibly across the entire genus.

Comments

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

© 2018 Schafran et al.

Original Publication Citation

Schafran, P. W., Johnson, G., Taylor, W. C., Zimmer, E. A., & Musselman, L. J. (2018). Low‐copy nuclear markers in Isoëtes (Isoëtaceae) identified with transcriptomes. Applications in Plant Sciences, 6(4), e1142. doi:10.1002/aps3.1142

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