Document Type

Article

Publication Date

2014

DOI

10.1016/j.ttbdis.2013.08.003

Publication Title

Ticks and Tick-Borne Diseases

Volume

5

Issue

1

Pages

48-52

Abstract

Ixodes affinis Neumann (1899) and Ixodes scapularis Say (1821) are tick vectors of the etiologic agent of Lyme disease, Borrelia burgdorferi sensu stricto. Ixodes affinis and I. scapularis are morphologically very similar, and as they are sympatric in the mid- and south-Atlantic U.S. coastal states, their accurate identification is crucial to studies of disease and vector ecology in this area. This work describes a rapid, single-tube SYBR® Green-based real-time PCR assay for differentiation of I. affinis and I. scapularis at all life stages. The assay employs 2 pairs of species specific primers directed against the internal transcribed spacer 2 (ITS2) region of the nuclear rRNA operon. Amplification products for these primer pairs differ in size and may be differentiated with a melt curve analysis. This tool is intended as a supplement to morphological methods for accurate identification of these ticks.

Comments

NOTE: This is the author's manuscript version of a work that was published in Ticks and Tick-Borne Diseases. The final version was published as:

Wright, C. L., Hynes, W. L., White, B. T., Marshall, M. N., Gaff, H. D., & Gauthier, D. T. (2014). Single-tube real-time PCR assay for differentiation of Ixodes affinis and Ixodes scapularis. Ticks and Tick-Borne Diseases, 5(1), 48-52. doi:10.1016/j.ttbdis.2013.08.003

Available at: 10.1016/j.ttbdis.2013.08.003

Original Publication Citation

Wright, C. L., Hynes, W. L., White, B. T., Marshall, M. N., Gaff, H. D., & Gauthier, D. T. (2014). Single-tube real-time PCR assay for differentiation of Ixodes affinis and Ixodes scapularis. Ticks and Tick-Borne Diseases, 5(1), 48-52. doi:10.1016/j.ttbdis.2013.08.003

ORCID

0000-0003-3998-7781 (Hynes), 0000-0002-4034-2684 (Gaff)

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