Document Type

Article

Publication Date

7-2020

DOI

10.1371/journal.pgen.1008856

Publication Title

PLoS Genetics

Volume

16

Issue

7

Pages

e1008856 (24 pp.)

Abstract

The microRNAs (miRNAs) are important regulators of gene expression. In this study, we provide evidence for the first time to show that rickettsial pathogen Anaplasma phagocytophilum infection results in the down-regulation of tick microRNA-133 (miR-133), to induce Ixodes scapularis organic anion transporting polypeptide (isoatp4056) gene expression critical for this bacterial survival in the vector and for its transmission to the vertebrate host. Transfection studies with recombinant constructs containing transcriptional fusions confirmed binding of miR-133 to isoatp4056 mRNA. Treatment with miR-133 inhibitor resulted in increased bacterial burden and isoatp4056 expression in ticks and tick cells. In contrast, treatment with miR-133 mimic or pre-mir-133 resulted in dramatic reduction in isoatp4056 expression and bacterial burden in ticks and tick cells. Moreover, treatment of ticks with pre-mir-133 affected vector-mediated A. phagocytophilum infection of murine host. These results provide novel insights to understand impact of modulation of tick miRNAs on pathogen colonization in the vector and their transmission to infect the vertebrate host.

Comments

Copyright: © 2020 Ramasamy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original Publication Citation

Ramasamy, E., Taank, V., Anderson, J. F., Sultana, H., & Neelakanta, G. (2020). Repression of tick microRNA-133 induces organic anion transporting polypeptide expression critical for Anaplasma phagocytophilum survival in the vector and transmission to the vertebrate host. PLoS Genetics, 16(7), Article e1008856. https://doi.org/10.1371/journal.pgen.1008856

ORCID

0000-0002-2739-8229 (Ramasamy), 0000-0001-6290-4150 (Taank), 0000-0002-5682-3108 (Sultana), 0000-0001-8374-9604 (Neelakanta)

Share

Article Location

 
COinS