Extracellular vesicles are thought to facilitate pathogen transmission from arthropods to humans and other animals. Here, we reveal that pathogen spreading from arthropods to the mammalian host is multifaceted. Extracellular vesicles from Ixodes scapularis enable tick feeding and promote infection of the mildly virulent rickettsial agent Anaplasma phagocytophilum through the SNARE proteins Vamp33 and Synaptobrevin 2 and dendritic epidermal T cells. However, extracellular vesicles from the tick Dermacentor andersoni mitigate microbial spreading caused by the lethal pathogen Francisella tularensis. Collectively, we establish that tick extracellular vesicles foster distinct outcomes of bacterial infection and assist in vector feeding by acting on skin immunity. Thus, the biology of arthropods should be taken into consideration when developing strategies to control vector-borne diseases.
© 2021 The Authors
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Publisher's version available at: https://doi.org/10.1038/s41467-021-23900-8
Article states: "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE99 partner repository with the dataset identifier PXD018779 under the project name “Label free proteomics profiling of nanovesicle isolated from cultured salivary glands isolated from partially fed adult female Ixodes scapularis” – Project https://doi.org/10.6019/PXD018779. All other data are available upon reasonable request. Raw data from all experiments are available as Source Data file. Source data are provided with this paper."
Corresponding author: Joao H. F. Pedra (ORCID: 0000-0001-8740-2667)
Supplementary information is available online at: https://doi.org/10.1038/s41467-021-23900-8
Original Publication Citation
Oliva Chávez, A. S., Wang, X., Marnin, L., Archer, N. K., Hammond, H. L., Carroll, E. E. M., . . . Pedra, J. H. F. (2021). Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection. Nature Communications, 12(1), Article 3696, 1-17. https://doi.org/10.1038/s41467-021-23900-8
Oliva Chávez, Adela S.; Wang, Xiaowei; Marnin, Liron; Archer, Nathan K.; Hammond, Holly L.; McClure Carroll, Erin E.; Shaw, Dana K.; Tully, Brenden G.; Buskirk, Amanda D.; Ford, Shelby L.; Butler, L. Rainer; Shahi, Preeti; Morozova, Kateryna; Clement, Cristina C.; Lawres, Lauren; O'Neal, Anya J.; Mamoun, Choukri Ben; Mason, Kathleen L.; Hobbs, Brandi E.; Scoles, Glen A.; Barry, Eileen M.; Sonenshine, Daniel E.; Pal, Utpal; Valenzuela, Jesus G.; Sztein, Marcelo B.; Pasetti, Marcela F.; Levin, Michael L.; Kotsyfakis, Michail; Jay, Steven M.; Huntley, Jason F.; Miller, Lloyd S.; Santambrogio, Laura; and Pedra, Joao H.F., "Tick Extracellular Vesicles Enable Arthropod Feeding and Promote Distinct Outcomes of Bacterial Infection" (2021). Biological Sciences Faculty Publications. 447.