Document Type
Article
Publication Date
2024
DOI
10.1016/j.xjon.2024.12.003
Publication Title
JTCVS Open
Volume
Article in Press
Pages
21 pp.
Abstract
Objective
Atrial cryolesions are usually created from the endocardium with the heart arrested. Some cardiac surgeons have used cryoablation epicardially during cardiopulmonary bypass, which is convenient because it does not require an incision into the atrial wall. Here, we analyzed the transmurality of epicardial cryoablations created during cardiopulmonary bypass in an arrested heart 30 days after ablation.
Methods
In Yucatan minipigs (n=5), hearts were exposed via sternotomy. Both caval veins were cannulated to collect blood for the cardiopulmonary bypass. Cryolesions were created applying a cryoprobe for 4 minutes per lesion. Hearts were harvested 30 days after the surgery. The transmurality of each lesion was determined for three transversal sections using triphenyl tetrazolium chloride (TTC) staining. The structure of the lesions was assessed with Gomori trichrome stains.
Results
We created a total of 20 atrial lesions and stained 175 sections. Transmurality was achieved in 96% of the sections and 75% of lesions. A significant fraction of the sections (12%) was transmural but had a width of less than 1.5 mm.
Conclusions
While epicardial cryoablation during bypass achieved better results than epicardial cryoablation of beating hearts, it falls short of conventional cryoablation and RF ablation.
Rights
© 2024 The Authors.
This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC-BY-NC-ND 4.0).
Original Publication Citation
Serra, F., Philpott, J. M., Bulysheva, A., & Zemlin, C. W. (2024). Epicardial cryoablation during cardiopulmonary bypass in a pig survival model. JTCVS Open. Advance online publication. https://doi.org/10.1016/j.xjon.2024.12.003
Repository Citation
Serra, Federica; Philpott, Jonathan M.; Bulysheva, Anna; and Zemlin, Christian W., "Epicardial Cryoablation During Cardiopulmonary Bypass in a Pig Survival Model" (2024). Electrical & Computer Engineering Faculty Publications. 499.
https://digitalcommons.odu.edu/ece_fac_pubs/499
Included in
Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Cardiology Commons, Cardiovascular System Commons