ORCID

https://orcid.org/0000-0002-1289-5302

College

College of Engineering & Technology (Batten)

Department

Biomedical Engineering

Graduate Level

Doctoral

Graduate Program/Concentration

Biomedical Engineering

Publication Date

2022

DOI

10.25883/hznb-va58

Abstract

Prior work has shown that our bioprinting system can reliably produce human mammary organoids and tumoroids with high precision. However, this was not previously applied to mouse models, which are also important with respect to translational research in cancer drug development. To address this, we have produced protocols for the development of in vitro structures from murine mammary epithelial and tumor cells. Additionally, we assessed the translatability of both human and murine bioprinted organoids into mouse mammary fat pads over a period of 6 weeks. Our lab found that our produced organoids are reliable, they can survive in vivo, and meaningfully integrate within host systems. Therefore, we have demonstrated that our system is adaptable to both human and murine models, as it offers a unique methodology for in vivo transplantation of human or murine organoids into mice, which can boost research efforts in cancer therapy research.

Keywords

Bioprinting, Organoid, Cancer, Tumoroid, Murine, Human, Translation, Implantation

Disciplines

Biological Engineering | Biomaterials | Biomedical Devices and Instrumentation | Medical Pathology | Molecular Biology | Molecular, Cellular, and Tissue Engineering | Systems and Integrative Engineering | Translational Medical Research

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3D Bioprinting and Implantation of Mouse Mammary Epithelial Structures Using a Custom Accessible 3D Bioprinting Platform


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