Nanosecond Pulsed Plasma And Electric Field Against Cancer Cells In Vitro

Author ORCiD

0000-0002-2932-9470

College

College of Engineering & Technology (Batten)

Department

Electrical & Computer Engineering

Graduate Level

Doctoral

Graduate Program/Concentration

Biomedical Engineering -- Bioelectrics

Presentation Type

No Preference

Abstract

Nanosecond pulsed plasma jet (ns-APPJ) is a form of gas discharge at atmospheric pressure that produces reactive agents at room temperature for cancer cell inactivation. These agents include charged particles, electric fields, and reactive oxygen and nitrogen species (ROS/RNS), which are known to induce oxidative stress in a biological system and can be applied for cancer therapy. Similarly, nanosecond pulsed electric field (nsPEF) have been studied extensively for tumor ablation because of its effect in cell membrane permeabilization and inducing apoptosis or regulated cell death. We have previously shown that synergistic treatments of a nanosecond pulsed plasma jet (ns-APPJ) and nsPEF enhance the inactivation effect on pancreatic cancer cells (Pan02)[1,2]. This study further examines the effects of the ns-APPJ and nsPEF on Pan02 cells in vitro. The intracellular ROS and loss of membrane potential induced by the ns-APPJ and nsPEF are evaluated with respect to the cell inactivation using fluorescent microscopy and flow cytometry. In addition, the roles of gas phase ROS produced by the ns-APPJ are assessed using optical emission spectroscopy (OES), and the mechanisms of synergistic effect observed are explored with proteomic analysis and further validated with Western Blot assay.

1. C. Jiang et al., "Synergistic effects of an atmospheric-pressure plasma jet and pulsed electric field on cells and skin," in IEEE Transactions on Plasma Science, 49, 11 (2021).

2. E. Oshin, et al., “Synergistic Effect of nanosecond pulsed plasma and electric field on inactivation of pancreatic cancer cells in vitro”. Sci Rep 14, 885 (2024).

Keywords

Biomedical engineering, medicine, cancer, applied physics, electrical and electronics engineering, plasma physics

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Nanosecond Pulsed Plasma And Electric Field Against Cancer Cells In Vitro

Nanosecond pulsed plasma jet (ns-APPJ) is a form of gas discharge at atmospheric pressure that produces reactive agents at room temperature for cancer cell inactivation. These agents include charged particles, electric fields, and reactive oxygen and nitrogen species (ROS/RNS), which are known to induce oxidative stress in a biological system and can be applied for cancer therapy. Similarly, nanosecond pulsed electric field (nsPEF) have been studied extensively for tumor ablation because of its effect in cell membrane permeabilization and inducing apoptosis or regulated cell death. We have previously shown that synergistic treatments of a nanosecond pulsed plasma jet (ns-APPJ) and nsPEF enhance the inactivation effect on pancreatic cancer cells (Pan02)[1,2]. This study further examines the effects of the ns-APPJ and nsPEF on Pan02 cells in vitro. The intracellular ROS and loss of membrane potential induced by the ns-APPJ and nsPEF are evaluated with respect to the cell inactivation using fluorescent microscopy and flow cytometry. In addition, the roles of gas phase ROS produced by the ns-APPJ are assessed using optical emission spectroscopy (OES), and the mechanisms of synergistic effect observed are explored with proteomic analysis and further validated with Western Blot assay.

1. C. Jiang et al., "Synergistic effects of an atmospheric-pressure plasma jet and pulsed electric field on cells and skin," in IEEE Transactions on Plasma Science, 49, 11 (2021).

2. E. Oshin, et al., “Synergistic Effect of nanosecond pulsed plasma and electric field on inactivation of pancreatic cancer cells in vitro”. Sci Rep 14, 885 (2024).