Date of Award

Fall 12-2020

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Biological Sciences

Program/Concentration

Biomedical Sciences

Committee Director

Hameeda Sultana

Committee Member

Girish Neelakanta

Committee Member

Dayle A. Daines

Committee Member

Olga Pakhomova

Abstract

Vector control is a global concern, and effective strategies have been on the way. Among the many different strategies, insect repellents to protect us from blood-feeding arthropods are very common. Food processing or preservatives in food expose us to several chemicals associated with insect repellents, insecticides, and pesticides. Using metallic conjugation with plant extracts or essential oils has become a widespread strategy to increase their efficacy. But it is worth it to be aware that some of those metallic compounds like Cobalt or Copper upon conjugation with thiosemicarbazone ligands derived from Citral and 2-acetylethiazole at the center coordination enhance both Dengue (DENV2; serotype 2) and Zika (ZIKV) replication. This study successfully demonstrated that under any tested conditions (pre or post-exposure), metal complexes with conjugation enhanced flaviviral replications in human skin keratinocytes (HaCaT cells). HeLa cells and primary cultures of mouse bone marrow-derived dendritic cells. These observations suggest that both human and animal cells are highly susceptible to flaviviral infection when exposed to coordinated metal conjugated compounds. In contrast, viral replication in mosquito (C6/36) cells was not considerably affected when exposed to conjugated metallic compounds. These data suggest that mosquitoes have better resistance and protection against such coordinated metal conjugated compounds and their ability to enhance DENV2 and ZIKV infection.

DOI

10.25777/35gg-zd36

ISBN

9798557053440

Available for download on Friday, December 23, 2022

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