Shape is an intrinsic marker of cell cycle, an important factor for identifying a bioparticle, and also a useful indicator of cell state for disease diagnostics. Therefore, shape can be a specific marker in label-free particle and cell separation for various chemical and biological applications. We demonstrate in this work a continuous-flow electrical sorting of spherical and peanut-shaped particles of similar volumes in an asymmetric double-spiral microchannel. It exploits curvature-induced dielectrophoresis to focus particles to a tight stream in the first spiral without any sheath flow and subsequently displace them to shape-dependent flow paths in the second spiral without any external force. We also develop a numerical model to simulate and understand this shape-based particle sorting in spiral microchannels. The predicted particle trajectories agree qualitatively with the experimental observation. (C) 2014 AIP Publishing LLC.
Original Publication Citation
DuBose, J., Lu, X. Y., Patel, S., Qian, S. Z., Joo, S. W., & Xuan, X. C. (2014). Microfluidic electrical sorting of particles based on shape in a spiral microchannel. Biomicrofluidics, 8(1), 0141011-0141018). doi:10.1063/1.4862355
DuBose, John; Lu, Xinyu; Patel, Saurin; Qian, Shizhi; and Joo, Sang Woo, "Microfluidic Electrical Sorting of Particles Based on Shape in a Spiral Microchannel" (2014). Mechanical & Aerospace Engineering Faculty Publications. 28.