Aim: To identify differential methylation related to prescribed opioid use. Methods: This study examined whether blood DNA methylation, measured using Illumina arrays, differs by recent opioid medication use in four population-based cohorts. We meta-analyzed results (282 users; 10,560 nonusers) using inverse-variance weighting. Results: Differential methylation (false discovery rate <0.05) was observed at six CpGs annotated to the following genes: KIAA0226, CPLX2, TDRP, RNF38, TTC23 and GPR179. Integrative epigenomic analyses linked implicated loci to regulatory elements in blood and/or brain. Additionally, 74 CpGs were differentially methylated in males or females. Methylation at significant CpGs correlated with gene expression in blood and/or brain. Conclusion: This study identified DNA methylation related to opioid medication use in general populations. The results could inform the development of blood methylation biomarkers of opioid use.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.
Article states: To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/epi-2022-0353.
Data sharing statement: Complete meta-analysis results can be found at Zenodo 10.5281/zenodo.7545108.
Original Publication Citation
Lee, M., Joehanes, R., McCartney, D. L., Kho, M., Hüls, A., Wyss, A. B., Liu, C., Walker, R. M., SL, R. K., Wingo, T. S., Burkholder, A., Ma, J., Campbell, A., Wingo, A. P., Huan, T., Sikdar, S., Keshawarz, A., Bennett, D. A., Smith, J. A., . . . London, S. J. (2022). Opioid medication use and blood DNA methylation: Epigenome-wide association meta-analysis. Epigenomics, 14(23), 1479-1492. https://doi.org/10.2217/epi-2022-0353
Lee, Mikyeong; Joehanes, Roby; McCartney, Daniel L.; Kho, Minjung; Hüls, Anke; Wyss, Annah B.; Liu, Chunyu; Walker, Rosie M.; Kardia, Sharon L.R.; Wingo, Thomas S.; Burkholder, Adam; Ma, Jiantao; Campbell, Archie; Wingo, Aliza P.; Huan, Tianxiao; Sikdar, Sinjini; Keshawarz, Amena; Bennett, David A.; Smith, Jennifer A.; Evans, Kathryn L.; Levy, Daniel; and London, Stephanie J., "Opioid Medication Use And Blood DNA Methylation: Epigenome-Wide Association Meta-Analysis" (2022). Mathematics & Statistics Faculty Publications. 223.
Published in print as part of Epigenomics Volume 14, Issue 23, in December 2022.
Online version published in January, 2023.