Document Type
Article
Publication Date
2015
DOI
10.1016/j.biomaterials.2015.06.019
Publication Title
Biomaterials
Volume
64
Pages
98-107
Abstract
Bone grafting procedures are performed to treat wounds incurred during wartime trauma, accidents, and tumor resections. Endogenous mechanisms of repair are often insufficient to ensure integration between host and donor bone and subsequent restoration of function. We investigated the role that bone marrow-derived cells play in bone regeneration and sought to increase their contributions by functionalizing bone allografts with bioactive lipid coatings. Polymer-coated allografts were used to locally deliver the immunomodulatory small molecule FTY720 in tibial defects created in rat bone marrow chimeras containing genetically-labeled bone marrow for monitoring cell origin and fate. Donor bone marrow contributed significantly to both myeloid and osteogenic cells in remodeling tissue surrounding allografts. FTY720 coatings altered the phenotype of immune cells two weeks post-injury, which was associated with increased vascularization and bone formation surrounding allografts. Consequently, degradable polymer coating strategies that deliver small molecule growth factors such as FTY720 represent a novel therapeutic strategy for harnessing endogenous bone marrow-derived progenitors and enhancing healing in load-bearing bone defects.
Original Publication Citation
Das, A., Segar, C. E., Chu, Y. H., Wang, T. W., Lin, Y., Yang, C. X., . . . Botchwey, E. A. (2015). Bioactive lipid coating of bone allografts directs engraftment and fate determination of bone marrow-derived cells in rat GFP chimeras. Biomaterials, 64, 98-107. doi:10.1016/j.biomaterials.2015.06.019
Repository Citation
Das, Anusuya; Segar, Claire E.; Chu, Yihsuan; Wang, Tiffany W.; Lin, Yon; Yang, Chunxi; and Ogle, Roy C., "Bioactive Lipid Coating of Bone Allografts Direct Engraftment and Fate Determination of Bone Marrow-Derived Cells in Rat GFP Chimeras" (2015). Medical Diagnostics & Translational Sciences Faculty Publications. 26.
https://digitalcommons.odu.edu/medicaldiagnostics_fac_pubs/26
Included in
Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Biomedical Engineering and Bioengineering Commons, Orthopedics Commons
Comments
NOTE: This is the author's pre-print version of a work that was published in Biomaterials. The final version was published as:
Das, A., Segar, C. E., Chu, Y. H., Wang, T. W., Lin, Y., Yang, C. X., . . . Botchwey, E. A. (2015). Bioactive lipid coating of bone allografts directs engraftment and fate determination of bone marrow-derived cells in rat GFP chimeras. Biomaterials, 64, 98-107. doi:10.1016/j.biomaterials.2015.06.019