We compared growth kinetics of Prorocentrum donghaiense cultures on different nitrogen (N) compounds including nitrate, ammonium urea, glutamic acid, dialanine, and cynate. P. donghaiense exhibited standard Monod-type growth kinetics over a range of nitrogen concentrations for all N. Compounds tested. Cultures grown on glu and urea had the highest maximum growth rates. However, cultures grown on cyanate had lower half saturation constants. Nitrogen uptake kinetics were measured in nitrate-deplete and replete batch cultures of P. donghaiense. In nitrate-deplete batch cultures, P. donghaiense exhibited Michaelis-Menten type uptake kinetics for nitrate, ammonium, urea and algal amino acids; uptake was saturated at or below 50 umol N L-1. In nitrate replete batch cultures, ammonium, urea, and algal amino acid uptake kinetics were similar to those measured in nitrate deplete batch cultures. Together, our results demonstrate that P. donghaiense can grow well on a variety of N sources, and exhibits similar uptake kinetics under both nutrient replete and deplete conditions. This may be an important factor facilitating their growth during bloom initiation and development in N-enriched estuaries where many algae compete for bioavailable N. and nutrient environment changes as a result of algal growth.
Original Publication Citation
Hu, Z. X., Duan, S. S., Xu, N., & Mulholland, M. R. (2014). Growth and nitrogen uptake kinetics in cultured Prorocentrum donghaiense. PLoS One, 9(4). doi: 10.1371/journal.pone.0094030
Hu, Zhangxi; Duan, Shunshan; and Mulholland, Margaret R., "Growth and Nitrogen Uptake Kinetics in Cultured Procentrum donghaiense" (2014). OES Faculty Publications. 19.