Document Type

Article

Publication Date

11-2019

DOI

10.1038/s41597-019-0292-2

Publication Title

Scientific Data

Volume

6

Pages

277 (8 pg.)

Abstract

SeaFlow is an underway flow cytometer that provides continuous shipboard observations of the abundance and optical properties of small phytoplankton (<5 μm in equivalent spherical diameter, ESD). Here we present data sets consisting of SeaFlow-based cell abundance, forward light scatter, and pigment fluorescence of individual cells, as well as derived estimates of ESD and cellular carbon content of picophytoplankton, which includes the cyanobacteria Prochlorococcus, Synechococcus and small-sized Crocosphaera (<5 μm ESD), and picophytoplankton and nanophytoplankton (2–5 μm ESD). Data were collected in surface waters (≈5 m depth) from 27 oceanographic cruises carried out in the Northeast Pacific Ocean between 2010 and 2018. Thirteen cruises provide high spatial resolution (≈1 km) measurements across 32,500 km of the Northeast Pacific Ocean and 14 near-monthly cruises beginning in 2015 provide seasonal distributions at the long-term sampling site (Station ALOHA) of the Hawaii Ocean Time-Series. These data sets expand our knowledge of the current spatial and temporal distributions of picophytoplankton in the surface ocean.

Rights

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

© The Author(s) 2019

Data Availability

Article states: "The dataset is a compilation of data assembled from diferent research cruises conducted since 2010. Each data record represents the cell abundance, median, 25% and 75% percentile of optical properties (chlorophyll and phycoerythrin fuorescence, forward scatter), ESD and carbon quotas for each population estimated at a certain point in space and time. Each data record belongs to a cruise, with cruise identifcation retrieved from the Rolling Deck to Repository, and is linked to its associated metadata such as time, location, depth, sea surface temperature and salinity, and PAR. Online-only Table 1 lists the variables, their defnition and units. The dataset is accessible as a.csv fle through Zenodo open access research data repository13.

Raw SeaFlow data are analyzed using our custom R package available on Github at https://github.com/armbrustlab/popcycle. The repository also includes a tutorial on the use of the sofware. Additional Github repositories are available for the virtual-core calibration, conversion of light scattering to cell size and conversion of light scattering to carbon quotas."

Comments

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

© The Author(s) 2019

Original Publication Citation

Ribalet, F., Berthiaume, C., Hynes, A., Swalwell, J., Carlson, M., Clayton, S., . . . Armbrust, E. V. (2019). SeaFlow data v1, high-resolution abundance, size and biomass of small phytoplankton in the North Pacific. Scientific Data, 6, 277. doi:10.1038/s41597-019-0292-2

ORCID

0000-0001-7473-4873 (Clayton)

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