Document Type

Article

Publication Date

12-2019

DOI

10.1038/s41597-019-0308-y

Publication Title

Scientific Data

Volume

6

Pages

303 (1-6)

Abstract

The heterotrophic marine bacterium, Ruegeria pomeroyi, was experimentally cultured under environmentally realistic carbon conditions and with a tracer-level addition of 13C-labeled leucine to track bacterial protein biosynthesis through growth phases. A combination of methods allowed observation of real-time bacterial protein production to understand metabolic priorities through the different growth phases. Over 2000 proteins were identified in each experimental culture from exponential and stationary growth phases. Within two hours of the 13C-labeled leucine addition, R. pomeroyi significantly assimilated the newly encountered substrate into new proteins. This dataset provides a fundamental baseline for understanding growth phase differences in molecular physiology of a cosmopolitan marine bacterium.

Comments

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files associated with this article.

© The Author(s) 2019

Original Publication Citation

Krayushkina, D., Timmins-Schiffman, E., Faux, J., May, D. H., Riffle, M., Harvey, H. R., & Nunn, B. L. (2019). Growth phase proteomics of the heterotrophic marine bacterium Ruegeria pomeroyi. Scientific Data, 6, 303. doi:10.1038/s41597-019-0308-y

ORCID

0000-0001-7746-7758 (Harvey)

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