Title

Differential Expression of Developmental and Signaling Genes Between Normal Chondrocytes and Chondrosarcoma Cells

Presenting Author Name/s

Arooba Ayaz

Faculty Advisor

Chris Osgood

Presentation Type

Oral Presentation

Disciplines

Cancer Biology | Cell Biology | Genetics

Description/Abstract

Introduction

Resistant to both radiation and chemotherapy, chondrosarcomas can be difficult to treat. Expression levels of two developmental genes, SOX5 and SOX6 were studied in chondrosarcoma cells and compared to normal chondrocytes. The expression levels of GPR68 which is involved mediation of growth inhibition and the expression of KCNN-2 which plays a role in cellular proliferation were also studied. Chondrosarcomas are thought to arise from mesenchymal stem cells, thus it is important to study the expression levels of genes that may be involved in the process of normal chondrogenesis and proliferation.

Methods

Both chondrosarcoma cells and normal chondrocytes were cultured in monolayer and pellet (3D) cultures, followed by RNA isolations and conversion to cDNA. RT-qPCR was used to characterize the expression levels of the genes and the ∆∆Ct method to quantify fold change.

Results

SOX5, SOX6, GPR68, and KCNN-2 were found to be expressed at lower levels (-11.04, -67.04, -294.41 respectively) in the chondrosarcoma cells when compared to normal chondrocytes.

Conclusions

Lower expression of SOX6 likely influences the expression of aggrecan in chondrosarcomas. Lower levels of GPR68 implies a lack of mediation of growth inhibition in these cancer cells as well as lower levels of intracellular calcium. Less expression of KCNN-2 probably alters the membrane potential of the chondrocytes which effects the proliferation of the cells. Overall, the data suggests that when compared to normal chondrocytes there is lower expression of SOX5, SOX6, GPR68 and KCNN-2 in chondrosarcoma cells which likely has effects on their growth and proliferation.

Session Title

Biological Sciences 1 Presentations

Location

Learning Commons @ Perry Library Conference Room 1310

Start Date

3-2-2018 9:00 AM

End Date

3-2-2018 10:00 AM

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Feb 3rd, 9:00 AM Feb 3rd, 10:00 AM

Differential Expression of Developmental and Signaling Genes Between Normal Chondrocytes and Chondrosarcoma Cells

Learning Commons @ Perry Library Conference Room 1310

Introduction

Resistant to both radiation and chemotherapy, chondrosarcomas can be difficult to treat. Expression levels of two developmental genes, SOX5 and SOX6 were studied in chondrosarcoma cells and compared to normal chondrocytes. The expression levels of GPR68 which is involved mediation of growth inhibition and the expression of KCNN-2 which plays a role in cellular proliferation were also studied. Chondrosarcomas are thought to arise from mesenchymal stem cells, thus it is important to study the expression levels of genes that may be involved in the process of normal chondrogenesis and proliferation.

Methods

Both chondrosarcoma cells and normal chondrocytes were cultured in monolayer and pellet (3D) cultures, followed by RNA isolations and conversion to cDNA. RT-qPCR was used to characterize the expression levels of the genes and the ∆∆Ct method to quantify fold change.

Results

SOX5, SOX6, GPR68, and KCNN-2 were found to be expressed at lower levels (-11.04, -67.04, -294.41 respectively) in the chondrosarcoma cells when compared to normal chondrocytes.

Conclusions

Lower expression of SOX6 likely influences the expression of aggrecan in chondrosarcomas. Lower levels of GPR68 implies a lack of mediation of growth inhibition in these cancer cells as well as lower levels of intracellular calcium. Less expression of KCNN-2 probably alters the membrane potential of the chondrocytes which effects the proliferation of the cells. Overall, the data suggests that when compared to normal chondrocytes there is lower expression of SOX5, SOX6, GPR68 and KCNN-2 in chondrosarcoma cells which likely has effects on their growth and proliferation.