Title

Pattern Detection in Three-Dimensional Cryo-EM Images of Filament Bundles

Presenting Author Name/s

Peter Scheible

Faculty Advisor

Jing He

Presentation Type

Oral Presentation

Disciplines

Bioinformatics

Description/Abstract

Cryo-electron microscopy is a fast-developing technology with many applications in structural biology analysis. Since most biological specimens are radiosensitive, they must be imaged using low doses of radiation. More radiation produces higher resolution images but risks causing radiation damage to the specimen. Low dose imaging produces noisier images that are often difficult to analyze. The purpose of this project is to develop an effective image processing tool using the nature of filaments in a bundle. By using cross-correlation and convolution on cross sections of the filament images, we are able to detect the overall direction of the bundle that can be utilized in downstream detection of individual filaments.

Session Title

Biological Sciences 2 Presentations

Location

Learning Commons @ Perry Library Conference Room 1310

Start Date

3-2-2018 10:15 AM

End Date

3-2-2018 11:15 AM

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Feb 3rd, 10:15 AM Feb 3rd, 11:15 AM

Pattern Detection in Three-Dimensional Cryo-EM Images of Filament Bundles

Learning Commons @ Perry Library Conference Room 1310

Cryo-electron microscopy is a fast-developing technology with many applications in structural biology analysis. Since most biological specimens are radiosensitive, they must be imaged using low doses of radiation. More radiation produces higher resolution images but risks causing radiation damage to the specimen. Low dose imaging produces noisier images that are often difficult to analyze. The purpose of this project is to develop an effective image processing tool using the nature of filaments in a bundle. By using cross-correlation and convolution on cross sections of the filament images, we are able to detect the overall direction of the bundle that can be utilized in downstream detection of individual filaments.