Title

Study of Effects of Dopamine on Embryonic Cardiovascular Development in Zebrafish

Presenting Author Name/s

Andriana C. Zourou

Faculty Advisor

Dr. X. Nancy Xu

Presentation Type

Oral Presentation

Disciplines

Behavioral Neurobiology | Biochemistry, Biophysics, and Structural Biology | Biology | Chemicals and Drugs | Developmental Biology

Description/Abstract

Zebrafish share genetic similarities to human and have served as an effective animal model to study a wide range of biological processes in human. Dopamine is a neurotransmitter that plays a central role in brain functions. However, the role of dopamine in the development of cardiovascular system and heart function is less clear. In this study, we used a transgenic zebrafish with a cardiac protein (Flk1) fused with green fluorescence protein (GFP) that enables us to directly visualize the Flk1 cardiac protein, and developing cardiovascular system in developing zebrafish embryos, and studied the effects of their development in the presence of various concentrations of dopamine. We characterized the dependence of heart rates, hatching and phenotypes of developed zebrafish upon the concentration of dopamine in vivo in real time using optical and fluorescence microscopy. Our results show the high dependence of cardiovascular development and heart function of developing embryos upon the concentration of dopamine.

Session Title

Chemistry

Location

Learning Commons @ Perry Library Conference Room 1310

Start Date

2-2-2019 11:30 AM

End Date

2-2-2019 12:30 PM

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Feb 2nd, 11:30 AM Feb 2nd, 12:30 PM

Study of Effects of Dopamine on Embryonic Cardiovascular Development in Zebrafish

Learning Commons @ Perry Library Conference Room 1310

Zebrafish share genetic similarities to human and have served as an effective animal model to study a wide range of biological processes in human. Dopamine is a neurotransmitter that plays a central role in brain functions. However, the role of dopamine in the development of cardiovascular system and heart function is less clear. In this study, we used a transgenic zebrafish with a cardiac protein (Flk1) fused with green fluorescence protein (GFP) that enables us to directly visualize the Flk1 cardiac protein, and developing cardiovascular system in developing zebrafish embryos, and studied the effects of their development in the presence of various concentrations of dopamine. We characterized the dependence of heart rates, hatching and phenotypes of developed zebrafish upon the concentration of dopamine in vivo in real time using optical and fluorescence microscopy. Our results show the high dependence of cardiovascular development and heart function of developing embryos upon the concentration of dopamine.