Title

Single Cell RNA Sequencing

Description/Abstract/Artist Statement

Studying gene activity in single cells is an important factor in understanding cell functions. Cell reaction is different depending on where the gene is expressed. This phenomenon happens because the structure of the cell determines its function. The concept of measuring gene activity and its changes in individual cells is referred to as RNA velocity. This concept has expanded the techniques of studying cellular differentiation. Using scVelo framework that was developed by Bergen et al. (Nature Biotechnology, 2020), we were able to obtain velocities using the ratios of spliced and un-spliced mRNA obtained from Velocyto framework, visualize velocities of each cell on a plot, track where each gene is expressed, and visualize which cells are spliced or un-spliced. Being able to use the scVelo framework will help with determining how cells functions with respect to each gene studied.

Presenting Author Name/s

Fozi Alkaifi

Faculty Advisor/Mentor

Jiangwen Sun

College Affiliation

College of Sciences

Presentation Type

Poster

Disciplines

Computer Sciences

Session Title

Poster Session

Location

Learning Commons @ Perry Library

Start Date

3-19-2022 9:00 AM

End Date

3-19-2022 11:00 AM

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Mar 19th, 9:00 AM Mar 19th, 11:00 AM

Single Cell RNA Sequencing

Learning Commons @ Perry Library

Studying gene activity in single cells is an important factor in understanding cell functions. Cell reaction is different depending on where the gene is expressed. This phenomenon happens because the structure of the cell determines its function. The concept of measuring gene activity and its changes in individual cells is referred to as RNA velocity. This concept has expanded the techniques of studying cellular differentiation. Using scVelo framework that was developed by Bergen et al. (Nature Biotechnology, 2020), we were able to obtain velocities using the ratios of spliced and un-spliced mRNA obtained from Velocyto framework, visualize velocities of each cell on a plot, track where each gene is expressed, and visualize which cells are spliced or un-spliced. Being able to use the scVelo framework will help with determining how cells functions with respect to each gene studied.