79 - High-Molecular Weight DNA Extraction and Nanopore Sequencing of PC-3 Cancer Cells for Genomic Analysis
Description/Abstract/Artist Statement
Prostate cancer is highly prevalent, affecting approximately 1 in 8 men throughout their lifetime. Advancing treatment options increasingly depends on comprehensive genomic analysis. Nanopore sequencing is a cutting-edge technology capable of generating ultra-long reads upwards of 100,000 base pairs, providing improved detection of structural variants, epigenetic modifications, and complex genomic rearrangements compared to traditional short-read sequencing methods. In this work, we cultured PC-3 prostate cancer cells and performed high-molecular weight DNA extraction, a technique to isolate and preserve long genomic fragments. The extracted DNA will be assessed for quality and size before being used for nanopore sequencing. The resulting read quality and coverage will also be assessed. Findings from this study may enhance our understanding of prostate cancer genomics, potentially informing biomarker discovery and therapeutic innovation in precision oncology.
Faculty Advisor/Mentor
Harold Riethman
Faculty Advisor/Mentor Department
School of Medical Diagnostic and Translational Sciences
College Affiliation
Ellmer College of Health Sciences
Presentation Type
Poster
Disciplines
Cancer Biology | Genomics | Molecular Biology
79 - High-Molecular Weight DNA Extraction and Nanopore Sequencing of PC-3 Cancer Cells for Genomic Analysis
Prostate cancer is highly prevalent, affecting approximately 1 in 8 men throughout their lifetime. Advancing treatment options increasingly depends on comprehensive genomic analysis. Nanopore sequencing is a cutting-edge technology capable of generating ultra-long reads upwards of 100,000 base pairs, providing improved detection of structural variants, epigenetic modifications, and complex genomic rearrangements compared to traditional short-read sequencing methods. In this work, we cultured PC-3 prostate cancer cells and performed high-molecular weight DNA extraction, a technique to isolate and preserve long genomic fragments. The extracted DNA will be assessed for quality and size before being used for nanopore sequencing. The resulting read quality and coverage will also be assessed. Findings from this study may enhance our understanding of prostate cancer genomics, potentially informing biomarker discovery and therapeutic innovation in precision oncology.