Event Title

The Temporal Dynamics of Caspase-3 Activation in the Extrinsic Process of Apoptosis

Location

Taylor 405, Madison Union, JMU

Start Date

4-6-2019 9:00 AM

Description

Apoptosis is a process of cell death that occurs within the body. Caspases are a family of enzymes which activate each other to degrade cellular proteins leading to the death of the cell. The steps of apoptosis are well known, however the timing of caspase activity within the cell is not. The purpose of this research is to understand the temporal dynamics of the caspase-3 activation in the extrinsic pathway of apoptosis. Cells are examined by fluorescence microscopy using an affinity microfluidic device and a fluorogenic caspase probe derived from Rhodamine 110. Fluorescence is monitored over a period of 6 hours, indicating the level of caspase activity within the cell. This procedure is conducted for caspase-3 using a DEVD2R probe. Caspase-3 activity was shown to start 5.3 hours post-induction lasting 20-40 minutes. Understanding the timing of caspase activation could be helpful in designing apoptosis-targeted therapies.

Presentation Type

Poster

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Apr 6th, 9:00 AM

The Temporal Dynamics of Caspase-3 Activation in the Extrinsic Process of Apoptosis

Taylor 405, Madison Union, JMU

Apoptosis is a process of cell death that occurs within the body. Caspases are a family of enzymes which activate each other to degrade cellular proteins leading to the death of the cell. The steps of apoptosis are well known, however the timing of caspase activity within the cell is not. The purpose of this research is to understand the temporal dynamics of the caspase-3 activation in the extrinsic pathway of apoptosis. Cells are examined by fluorescence microscopy using an affinity microfluidic device and a fluorogenic caspase probe derived from Rhodamine 110. Fluorescence is monitored over a period of 6 hours, indicating the level of caspase activity within the cell. This procedure is conducted for caspase-3 using a DEVD2R probe. Caspase-3 activity was shown to start 5.3 hours post-induction lasting 20-40 minutes. Understanding the timing of caspase activation could be helpful in designing apoptosis-targeted therapies.