Three-Dimensional Reconstructions of Tadpole Chondrocrania from Historical Sections

Authors

Gary Radice, University of Richmond
Mary K. Boggiano, University of Richmond
Mark DeSantis, University of Richmond
Peter Larson, University of Richmond
Joseph Oppong, University of Richmond
Matthew Smetanick, University of Richmond
Todd Stevens, University of Richmond
James Tripp, University of Richmond
Rebecca Weber, University of Richmond
Michael Kerckhove, University of Richmond
Rafael de Sá, University of Richmond

Document Type

Article

DOI

10.25778/11bs-k141

Abstract

Reconstructing three dimensional structures (3DR) from histological sections has always been difficult but is becoming more accessible with the assistance of digital imaging. We sought to assemble a low cost system using readily available hardware and software to generate 3DR for a study of tadpole chondrocrania. We found that a combination of RGB can1era, stereomicro­scope, and Apple Macintosh PowerPC computers running NIH Image, Object Image, Rotater, and SURFdriver software provided acceptable reconstruc­tions. These are limited in quality primarily by the distortions arising from histological protocols rather than hardware or software.

Recommended Citation

Radice, Gary; Boggiano, Mary K.; DeSantis, Mark; Larson, Peter; Oppong, Joseph; Smetanick, Matthew; Stevens, Todd; Tripp, James; Weber, Rebecca; Kerckhove, Michael; and Sá, Rafael de (1999) "Three-Dimensional Reconstructions of Tadpole Chondrocrania from Historical Sections," Virginia Journal of Science: Vol. 50: No. 3, Article 1.
DOI: 10.25778/11bs-k141
Available at: https://digitalcommons.odu.edu/vjs/vol50/iss3/1