Isolation and Fusion of Cotton Protoplasts

Authors

Michael H. Renfroe, James Madison University
Ronald C. Hartwig, Texas A&M University
Roberta H. Smith, Texas A&M University

Document Type

Article

DOI

10.25778/gq87-dz65

Abstract

Protoplasts were isolated from five species of Gossypium. Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated from cell suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. klotzschianum, G. harknessii, and G. herbaceum. Protoplasts of G. harknessii were enucleated and successfully fused with protoplasts of G. hirsutum.

Recommended Citation

Renfroe, Michael H.; Hartwig, Ronald C.; and Smith, Roberta H. (2001) "Isolation and Fusion of Cotton Protoplasts," Virginia Journal of Science: Vol. 52: No. 1, Article 5.
DOI: 10.25778/gq87-dz65
Available at: https://digitalcommons.odu.edu/vjs/vol52/iss1/5