Date of Award

Summer 1993

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry

Committee Director

Roy L. Williams

Committee Member

Laura K. Moen

Committee Member

Mark S. Elliot

Committee Member

Charles E. Bell

Call Number for Print

Special Collections LD4331.C45R43

Abstract

Grape seed extract is being marketed in France for cardiovascular use and skin care. The active constituents of both these drugs are certain polyphenols called procyanidins. A similar seed extract has now been obtained from Virginia Chardonnay grape seeds. Ten of the major components in this extract have been isolated in their pure form by the semi-preparative HPLC separation on a YMC ODS-AQ (Octadecylsilane-AQ) column. The ODS-Aq column has been shown to have a better resolving capacity compared to the Spherisorb ODS (Octadecylsilane) column previously described in the literature. This has been attributed to the ODSAQ support which has both a hydrophobic high carbon loading and a relatively hydrophilic surface. By using published HPLC and TLC methods, six procyanidin dimers and three trimers have been identified from this extract.

Treatment of human fibroblast cell cultures with several of these compounds has shown that they have a growth inhibitory effect on these cells. Similar treatment of HL-60 tumor cell lines with these same procyanidins did not have any inhibition on their growth pattern. Procyanidins are known free radical scavengers and the same fractions have been tested for their protective influence on fibroblast cells against free radical induced damage. Hypoxanthine/xanthine oxidase induced free radical damage was found to be greater with cells treated with procyanidins than with untreated cells grown in MEM/10% BS media. Studies with low levels of serum in media showed some protective influence of these compounds against free radical damage of fibroblast cells. They were also tested for their ability to inhibit xanthine oxidase. No inhibition of xanthine oxidase by procyanidins up to a concentration of 200 pM was observed.

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DOI

10.25777/jcqq-cf92

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