Document Type

Article

Publication Date

2010

Publication Title

Gene Therapy

Volume

17

Issue

3

Pages

419-423

DOI

10.1038/gt.2009.153

Abstract

Gene therapy is an attractive method for the treatment of cardiovascular disease. However, using current strategies, induction of gene expression at therapeutic levels is often inefficient. In this study, we show a novel electroporation (EP) method to enhance the delivery of a plasmid expressing an angiogenic growth factor (vascular endothelial growth factor, VEGF), which is a molecule previously documented to stimulate revascularization in coronary artery disease. DNA expression plasmids were delivered in vivo to the porcine heart with or without coadministered EP to determine the potential effect of electrically mediated delivery. The results showed that plasmid delivery through EP significantly increased cardiac expression of VEGF compared with injection of plasmid alone. This is the first report showing successful intracardiac delivery, through in vivo EP, of a protein expressing plasmid in a large animal.

Original Publication Citation

Marshall, W. G., Jr., Boone, B. A., Burgos, J. D., Gografe, S. I., Baldwin, M. K., Danielson, M. L., . . . Heller, R. (2010). Electroporation-mediated delivery of a naked DNA plasmid expressing VEGF to the porcine heart enhances protein expression. Gene Therapy, 17(3), 419-423. doi:10.1038/gt.2009.153

ORCID

0000-0003-1899-3859 (Heller, R.), 0000-0001-8084-0787 (Heller, L.)

Download

Share

COinS