Document Type
Article
Publication Date
7-2017
Publication Title
PLoS One
Volume
12
Issue
7
Pages
e0181002 (1-20)
DOI
10.1371/journal.pone.0181002
Abstract
This study examined the effect of 5 ns electric pulses on macroscopic ionic currents in whole-cell voltage-clamped adrenal chromaffin cells. Current-voltage (I-V) relationships first established that the early peak inward current was primarily composed of a fast voltage-dependent Na+ current (INa), whereas the late outward current was composed of at least three ionic currents: a voltage-gated Ca2+ current (ICa), a Ca2+-activated K+ current (IK(Ca)), and a sustained voltage-dependent delayed rectifier K+ current (IKV). A constant-voltage step protocol was next used to monitor peak inward and late outward currents before and after cell exposure to a 5 ns pulse. A single pulse applied at an electric (E)-field amplitude of 5 MV/m resulted in an instantaneous decrease of ~4% in peak INa that then declined exponentially to a level that was ~85% of the initial level after 10 min. Increasing the E-field amplitude to 8 or 10 MV/m caused a twofold greater inhibitory effect on peak INa. The decrease in INa was not due to a change in either the steady-state inactivation or activation of the Na+ channel but instead was associated with a decrease in maximal Na+ conductance. Late outward current was not affected by a pulse applied at 5 MV/m. However, for a pulse applied at the higher E-field amplitudes of 8 and 10 MV/m, late outward current in some cells underwent a progressive ~22% decline over the course of the first 20 s following pulse exposure, with no further decline. The effect was most likely concentrated on ICa and IK(Ca) as IKV was not affected. The results of this study indicate that in whole-cell patch clamped adrenal chromaffin cells, a 5 ns pulse differentially inhibits specific voltage-gated ionic currents in a manner that can be manipulated by tuning E-field amplitude.
Original Publication Citation
Yang, L., Craviso, G. L., Vernier, P. T., Chatterjee, I., & Leblanc, N. (2017). Nanosecond electric pulses differentially affect inward and outward currents in patch clamped adrenal chromaffin cells. PLoS One, 12(7), e0181002. doi:10.1371/journal.pone.0181002
Repository Citation
Yang, Lisha; Craviso, Gale L.; Vernier, P. Thomas; Chatterjee, Indira; and Leblanc, Normand, "Nanosecond Electric Pulses Differentially Affect Inward and Outward Currents in Patch Clamped Adrenal Chromaffin Cells" (2017). Bioelectrics Publications. 130.
https://digitalcommons.odu.edu/bioelectrics_pubs/130
ORCID
0000-0003-2335-1500 (Vernier)
Included in
Biomedical Engineering and Bioengineering Commons, Cell and Developmental Biology Commons
Comments
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.