Document Type

Article

Publication Date

2016

Publication Title

Bioelectrochemistry

Volume

110

Pages

1-12

DOI

10.1016/j.bioelechem.2016.02.011

Abstract

For this systematic review, 203 published reports on effects of electroporation using nanosecond high-voltage electric pulses (nsEP) on eukaryotic cells (human, animal, plant) in vitro were analyzed. A field synopsis summarizes current published data in the field with respect to publication year, cell types, exposure configuration, and pulse duration. Published data were analyzed for effects observed in eight main target areas (plasma membrane, intracellular, apoptosis, calcium level and distribution, survival, nucleus, mitochondria, stress) and an additional 107 detailed outcomes. We statistically analyzed effects of nsEP with respect to three pulse duration groups: A: 1–10 ns, B: 11–100 ns and C: 101–999 ns. The analysis confirmed that the plasma membrane is more affected with longer pulses than with short pulses, seen best in uptake of dye molecules after applying single pulses. Additionally, we have reviewed measurements of nsEP and evaluations of the electric fields to which cells were exposed in these reports, and we provide recommendations for assessing nanosecond pulsed electric field effects in electroporation studies.

Comments

This is an open access article under the CC BY-NC-ND license.

Original Publication Citation

Batista Napotnik, T., Reberšek, M., Vernier, P. T., Mali, B., & Miklavčič, D. (2016). Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro): A systematic review. Bioelectrochemistry, 110, 1-12. doi:https://doi.org/10.1016/j.bioelechem.2016.02.011

ORCID

0000-0003-2335-1500 (Vernier)

Download

Share

COinS