Document Type

Abstract

Publication Date

2015

Publication Title

Molecular Therapy

Volume

23

Pages

S145

DOI

10.1016/s1525-0016(16)33974-0

Abstract

[Introduction] Developing an optimal gene electro-transfer system for delivery of plasmid DNA to the skin in vivo has been challenging with expression often confined in the epithelium requiring high voltage electric fields, which can cause cellular and tissue damage. Minimizing such damage, while optimizing gene expression profiles, are highly desirable for therapeutic applications of gene delivery to the skin. Cell membrane fluidity is temperature dependent, thus moderate temperature elevation can increase membrane fluidity. Utilizing this concept, we developed a novel gene electro-transfer electrode for in vivo applications with ability to heat tissue with a laser integrated into a four pin multi-electrode array, to improve gene delivery efficiency.

Comments

Published under an Attribution-Noncommercial-NoDerivatives4.0 International license.

https://creativecommons.org/licenses/by-nc-nd/4.0/

Original Publication Citation

Bulysheva, A., Donate, A., Edelblute, C., Jung, D., Muhammad, M., Karl, S., & Richard, H. (2015). Increased tissue temperature improves electro-transfer mediated gene delivery to skin. Molecular Therapy, 23, S145. doi:10.1016/s1525-0016(16)33974-0

ORCID

0000-0002-0481-5675 (Donate)

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