Document Type
Article
Publication Date
2018
Publication Title
Cancer Cell International
Volume
18
Issue
42
Pages
1-11
DOI
10.1186/s12935-018-0541-z
Abstract
BACKGROUND: Despite new progress of chemotherapy in multiple myeloma (MM) clinical treatment, MM is still a refractory disease and new technology is needed to improve the outcomes and prolong the survival. Cold atmospheric plasma is a rapidly developed technology in recent years, which has been widely applied in biomedicine. Although plasma could efficiently inactivate various tumor cells, the effects of plasma on tumor cell metabolism have not been studied yet.
METHODS: In this study, we investigated the metabolite profiling of He plasma treatment on myeloma tumor cells by gas-chromatography time-of-flight (GC-TOF) mass-spectrometry. Meanwhile, by bioinformatic analysis such as GO and KEGG analysis we try to figure out the metabolism pathway that was significantly affected by gas plasma treatment.
RESULTS: By GC-TOF mass-spectrometry, 573 signals were detected and evaluated using PCA and OPLS-DA. By KEGG analysis we listed all the differential metabolites and further classified into different metabolic pathways. The results showed that beta-alanine metabolism pathway was the most significant change after He gas plasma treatment in myeloma cells. Besides, propanoate metabolism and linoleic acid metabolism should also be concerned during gas plasma treatment of cancer cells.
CONCLUSIONS: Cold atmospheric plasma treatment could significantly alter the metabolite profiling of myeloma tumor cells, among which, the beta-alanine metabolism pathway is the most susceptible to He gas plasma treatment. © The Author(s) 2018.
Original Publication Citation
Xu, D., Xu, Y., Ning, N., Cui, Q., Liu, Z., Wang, X., . . . Kong, M. G. (2018). Alteration of metabolite profiling by cold atmospheric plasma treatment in human myeloma cells. Cancer Cell Int, 18(42), 1-11. doi:10.1186/s12935-018-0541-z
Repository Citation
Xu, Dehui; Xu, Yujing; Ning, Ning; Cui, Qingjie; Liu, Zhijie; Wang, Xiaohua; Liu, Dingxin; Chen, Hailan; and Kong, Michael G., "Alteration of Metabolite Profiling by Cold Atmospheric Plasma Treatment in Human Myeloma Cells" (2018). Bioelectrics Publications. 161.
https://digitalcommons.odu.edu/bioelectrics_pubs/161
Included in
Cell Biology Commons, Molecular, Cellular, and Tissue Engineering Commons, Oncology Commons
Comments
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